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SDS–PAGE of the C.EcoO109I–Hisx6 obtained from E.coli HB101. Fractions containing C.EcoO109I–Hisx6 eluted from the His-Bind column were collected and stored at –20°C. A finely dispersed precipitate appearing on melting was removed by centrifugation, and the resulting clear solution (lane 2) and eluted fractions (lane 1) were separated on a 0.1% SDS–15% polyacrylamide gel. The positions of molecular mass standards (Mr) are indicated (in kDa) on the left.
