Figure 5.

Gel mobility shift assays with DNA fragments upstream of ecoO109IC and ecoO109IM. The substrate DNAs (I–V) indicated in Figure 3 were incubated with 9, 3, 1 and 0.33 pmol of purified C.EcoO109I–Hisx6, and then separated on a 8% polyacrylamide gel. The wedges indicate decreasing amounts of protein added.