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Interaction of C.EcoO109I–Hisx6 with various oligoduplexes. (A) DNA sequence upstream of the ecoO109IC translational start site. The base pairs constituting the 2-fold symmetrical sequences are boxed. (B) Oligonucleotides used for assaying. (C) Gel mobility shift analysis with oligoduplexes. Oligoduplexes were incubated with 1, 3 and 9 pmol of purified C.EcoO109I–Hisx6, and separated on a 12% polyacrylamide gel. The wedges indicate increasing amounts of protein added.
