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Gel mobility shift analysis with oligoduplexes with deletion (A) and insertion (B) between the inverted repeat. DNA sequences of the upper strand of oligoduplexes are shown, and the inverted repeat is underlined. Oligoduplexes of the wild-type and mutant sequences were incubated with 9 pmol of purified C.EcoO109I–Hisx6, and then separated on a 12% polyacrylamide gel.
