Figure 9.

Gel electrophoresis of permutated fragments of C.EcoO109I-binding sites. (A) pBend109 constructs and DNA fragments used for assaying. Oligonucleotides with a C.EcoO109I–Hisx6 binding site (shown by open bars), 5′-TCGACAAAAACTAAGTATTCCTTAGTAATT-3′ and 5′-CTAGAATTACTAAGGAATACTTAGTTTTTG-3′, were annealed and then inserted between the XbaI and SalI sites in pBend2 to obtain pBend109. 141-bp DNA fragments were excised from pBend109 with the restriction enzymes indicated above and used for DNA-binding assays. (B) DNA fragments were incubated with C.EcoO109I–Hisx6 and separated on a 8% polyacrylamide gel.