Figure 2.

URE mutations disrupt α-TM splicing. (A) URE sequences of mutant constructs. (B) PAC-1 SM cells and (C) HeLa cells were transiently transfected with wild-type reporter construct pTWT and various URE mutants. In lane MOCK no DNA was transfected into the cells. Harvested RNA was analysed by RT–PCR. Positions of the amplified bands corresponding to the 1–3–4 and 1–4 splicing patterns are indicated. The percentage of exon 3 skipping is shown immediately below each lane and represents the mean ± standard deviation of triplicate experiments.