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Substitution of the URE and DUGC elements. (A) Sequences at the URE and DUGC positions of each construct. UGC motifs are underlined. Bases in lower case in the downstream sequences represent additional BstXI compatible ends that were used to clone the URE sequence into the DUGC position. PAC-1 SM (B) and HeLa (C) cells were transiently transfected with URE substitution constructs and analysed as in Figure 2. The URE and DUGC elements of α-TM are not functionally interchangeable.
