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. 2002 Aug 15;30(16):e83. doi: 10.1093/nar/gnf082

Figure 4.

Figure 4

Counting of CAG repeats in fCAG30, fCAG35 and fCAG81 at an equimolar enzyme to substrate ratio. The DNA substrates fCAG30, fCAG35 and fCAG80 were Cy5-labeled at their 3′-ends. EcoP15I cleavage fragments were separated on non-denaturing 7% (w/v) polyacrylamide gels and analyzed using the ALFexpress DNA Analysis System. Output data show DNA fragments as fluorescence intensity F in arbitrary units. Cleavage by EcoP15I resulted in a ladder of DNA fragments in 3 bp steps from 139 to 223 bp for fCAG30, 139 to 238 bp for fCAG35 and 139 to 376 bp for fCAG81. In addition, a 117 bp DNA fragment was observed due to the cleavage at the inverse EcoP15I recognition site ‘i(3′)’.