Table 1. Oligonucleotide primers and synthetic DNA templates for detecting mutations in the p53 gene.

In the top table the sequences and the calculated masses of primers and the four possible single base extension products relative to the internal mass standard are listed. The bold numbers refer to the nucleotide variations detected in the p53 gene. In the bottom table the six nucleotide variations in templates 1 and 2 are shown in bold. Template 1 contains a heterozygous genotype (G/T).