Figure 2. Conservation of ABCA1 Amino Acid Position 1091 in Related Proteins and Functional Effect of Mutation at This Site.

(A) Multiple sequence alignment adapted from the view of family PTHR19229 available on the PANTHER Web site, showing the ABCA1, ABCA2, ABCA4, and ABCA7 subfamilies. Human ABCA1 position 1091 is highlighted in red; other conserved positions are highlighted in blue.

(B) Cholesterol efflux was assessed in 293 cells stably transfected with wild-type, M1091T, M1091L, or M1091V ABCA1 alleles. *p < 0.001.