Table 1.
Growth performance and glutamate production of Corynebacterium glutamicum CS176 under various oxygen levels, carbon sources, and biotin concentrations.
| Oxygen level | Carbon source | Biotin concentration (µg L-1) | Specific growth rate (umax, h-1) | Maximum growth (Klett unit) at culturing time (h) |
Glutamate production (mg L-1) |
|---|---|---|---|---|---|
| High | Glucose | 30 | 0.234 ± 0.009 | 463.00 ± 8.54 (20) | 131.27 ± 11.32 |
| 200 | 0.279 ± 0.015 | 465.00 ± 5.00 (20) | 122.97 ± 6.51 | ||
| Arabinose | 30 | 0.290 ± 0.010 | 485.00 ± 5.00 (36) | 39.98 ± 3.88 | |
| Glucose + Arabinose | 30 | 0.350 ± 0.016 | 555.00 ± 10.00 (30) | 463.27 ± 9.44 | |
| Medium high | Glucose | 0 | 0.302 ± 0.020 | 358.00 ± 9.85 (36) | 1,414.64 ± 37.71 |
| 0.5 | 0.341 ± 0.027 | 364.20 ± 5.19 (28) | 1,419.20 ± 19.05 | ||
| 1 | 0.332 ± 0.019 | 364.67 ± 9.50 (48) | 1,336.97 ± 37.60 | ||
| 3 | 0.347 ± 0.030 | 388.33 ± 10.41 (48) | 729.32 ± 12.90 | ||
| 30 | 0.348 ± 0.028 | 413.33 ± 14.43 (28) | 656.22 ± 7.36 | ||
| 200 | 0.290 ± 0.011 | 409.83 ± 5.01 (48) | 521.36 ± 16.76 | ||
| Arabinose | 30 | 0.309 ± 0.014 | 428.33 ± 7.64 (36) | 230.87 ± 6.422 | |
| Glucose + Arabinose | 30 | 0.303 ± 0.008 | 438.33 ± 7.64 (48) | 2,173.02 ± 20.93 | |
| Medium low | Glucose | 30 | 0.296 ± 0.009 | 332.64 ± 6.43 (36) | 2,007.03 ± 18.51 |
| 200 | 0.284 ± 0.011 | 318.33 ± 10.41 (48) | 1,857.63 ± 13.27 | ||
| Arabinose | 30 | 0.273 ± 0.006 | 373.33 ± 7.64 (36) | 654.16 ± 14.78 | |
| Glucose + Arabinose | 30 | 0.313 ± 0.019 | 338.37 ± 7.68 (48) | 2,521.60 ± 13.34 | |
| Low | Glucose | 30 | 0.283 ± 0.004 | 301.00 ± 3.61 (48) | 139.57 ± 7.08 |
| 200 | 0.236 ± 0.005 | 299.67 ± 3.21 (48) | 106.37 ± 8.65 | ||
| Arabinose | 30 | 0.261 ± 0.008 | 288.33 ± 12.58 (48) | 48.28 ± 6.86 | |
| merge this cell to “Low” | Glucose + Arabinose | 30 | 0.303 ± 0.001 | 286.67 ± 5.77 (48) | 122.97 ± 11.95 |
The data represent mean values ± standard deviations from replicate experiments.