Table 1.
Observed versus Random Frequencies of Backbone Cleavages Adjacent to Acidic (Asp and Glu), Basic (Arg, Lys, and His), and Proline Residues for All Charge States of the Proteins Dissociated by SORI-CAD in This Worka
acidic % | basic % | proline % | ||
---|---|---|---|---|
cytochrome c | observed | 30 | 45 | 7 |
random | 20 | 39 | 8 | |
myoglobin | observed | 38 | 36 | 6 |
random | 27 | 36 | 5 | |
carbonic anhydrase | observed | 15 | 10 | 33 |
random | 20 | 25 | 14 |
A total of 44, 47, and 39 backbone cleavages are observed for cytochrome c, myoglobin, and carbonic anhydrase, respectively.