Skip to main content
. Author manuscript; available in PMC: 2006 Jan 20.
Published in final edited form as: Anal Chem. 2004 Jul 15;76(14):3944–3950. doi: 10.1021/ac049724

Table 2.

Percentages of Analyte Ion Abundance Due to Adduction for Charge States of Cytochrome c (10−5 M) Formed from Aqueous Solutions Containing Different Concentrations of Ammonium Acetatea

[NH4Ac] (M) 9+ 8+ 7+ 6+
0 36 ± 8 29 ± 3 53 ± 8 <DL
1.0 × 10−3 25 ± 2 29 ± 6 72 ± 10 <DL
1.0 × 10−1 <DL 48 ± 15 76 ± 10 82 ± 5
8 <DL 20 ± 2 23 ± 5 26 ± 4
a

For 0, 1.0 × 10−3, and 1.0 × 10−1 M NH4Ac, the adducts are due to sodium (+22 Da; Na+ displaces H+). For 8 M NH4Ac, the adducts are due to ammonia (17 Da) and acetic acid (60 Da). Reported errors are ±1 standard deviation from the mean for three replicate measurements. <DL indicates that a given charge state is below detection limits.