Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| HeLa parental (H. sapiens) | ATCC | CCL-2 |
| HeLa cyclin B1-Venus (H. sapiens) | Pines Lab | |
| HeLa cyclin B1-Venus/H2B-mRFP (H. sapiens) | This study | |
| HeLa DHC-GFP (H. sapiens) | www.mitocheck.org | |
| HeLa POM121-3xGFP (H. sapiens) | Uhlmann Lab | |
| HeLa POM121-3xGFP/H2B-mRFP (H. sapiens) | This study | |
| RPE-1 cyclin B1-Venus (H. sapiens) |
Pines Lab Collin et al, 2013 |
|
| RPE-1 cyclin B1-eYFP (H. sapiens) |
Lindqvist Lab Akopyan et al, 2014 |
|
| Recombinant DNA | ||
| mRFP1-KASH-DN |
Gundersen Lab Gant Luxton et al, 2010 |
|
| mRFP1-KASH-DL |
Gundersen Lab Gant Luxton et al, 2010 |
|
| Antibodies | ||
| Mouse anti-Lamin A/C | Abcam | 4C11 |
| Rabbit anti-Lamin B1 | Abcam | ab16048 |
| Rat anti-alpha tubulin | Bio-Rad | MCA77G |
| Rabbit anti-NudE/NudEL antibody | Vallee Lab (Stehman et al, 2007) | |
| Rabbit anti-BicD2 | Atlas Antibodies | HPA024452 |
| Rabbit anti-SUN1 | Merck | HPA008346 |
| Rabbit anti-SUN2 | Merck | HPA001209 |
| Rabbit anti-SMC2 antibody | Bethyl Laboratories | A300-056A |
| Rabbit anti-cPLA2 | Cell Signaling | #2832 |
| Rabbit anti-CENP-F | Abcam | ab5 |
| Mouse monoclonal anti-p21WAF1 (Ab-1) | Merck | EA10 |
| Rabbit monoclonal anti-p38 (D13E1) | Cell Signaling Technology | 8690 |
| Rabbit monoclonal anti-Wee1 (D10D2) | Cell Signaling Technology | 13084 |
| Rabbit anti-CDK1 Y15 | Cell Signaling Technology | 9111 |
| Mouse anti-Nde1 | Abnova | H00054820-M01 |
| Rabbit anti-vinculin | ThermoFisher | 700062 |
| Rabbit monoclonal anti-acetyl-Histone H3 (Lys9) (C5B11) | Cell Signaling Technology | 9649 |
| Mouse monoclonal anti-Nesprin-2 (SYNE-2, F11) | Santa Cruz Biotechnology | sc-398616 |
| Goat anti-rabbit Alexa Fluor 488 | ThermoFisher | A-11008 |
| Goat anti-mouse Alexa Fluor 488 | ThermoFisher | A-11001 |
| Goat anti-rabbit Alexa Fluor 568 | ThermoFisher | A-11011 |
| Goat anti-mouse Alexa Fluor 568 | ThermoFisher | A-11004 |
| Goat anti-rat Alexa Fluor 568 | ThermoFisher | A-11077 |
| Goat anti-rat Alexa Fluor 647 | ThermoFisher | A-21247 |
| Goat anti-mouse Alexa Fluor 647 | ThermoFisher | A-21240 |
| Goat anti-mouse-HRP | Jackson Immuno Research | 115-005-003 |
| Goat anti-rabbit-HRP | Jackson Immuno Research | 111-005-003 |
| Goat anti-rat-HRP | Jackson Immuno Research | 112-005-003 |
| Oligonucleotides and other sequence-based reagents | ||
| Lamin A RNAi | Dharmacon ONTARGETplus | LQ-004978-00-0010 |
| BicD2 RNAi | Dharmacon ONTARGETplus | L-014060-01-0020 |
| SUN1 RNAi | Dharmacon ONTARGETplus | L-025277-01-0020 |
| SUN2 RNAi | Dharmacon ONTARGETplus | L-009959-01-0020 |
| NudE siRNA | Sigma-Aldrich |
5‘-GCUUGAAUCAGGCCAUCGA-3‘ 5‘-UCGAUGGCCUGAUUCAAGC-3‘ |
| NudEL siRNA | Sigma-Aldrich |
5‘-GGAUGAAGCAAGAGAUUUA3‘ 5‘-UAAAUCUCUUGCUUCAUCC-3‘ |
| SMC2 siRNA | Sigma-Aldrich | 5′-UGCUAUCACUGGCUUAAAUTT-3 |
| Non-targeting pool siRNA | Dharmacon | #D-001810-10-20 |
| Chemicals, enzymes and other reagents | ||
| ICRF-193 | Merck-Millipore | I4659 |
| Valproic acid (VPA), sodium salt | Sigma-Aldrich | P4543 |
| MK-1175 (Wee1 inhibitor) | MedChemExpress | HY-10993 |
| RO-3306 (CDK1 inhibitor) | Sigma-Aldrich | SML0569 |
| Thymidine | Sigma-Aldrich | T1895 |
| Dimethyl sulfoxide (DMSO) | Sigma-Aldrich | D4540 |
| Fibronectin (FBN) | Sigma-Aldrich | F1141 |
| Sodium Bicarbonate (NaHCO3) | ||
| Dulbecco’s Modified Eagle Medium (DMEM) | ThermoFisher | 11965092 |
| Opti-MEM Reduced Serum Medium | ThermoFisher | 11058021 |
| DMEM/F12 | ThermoFisher | 11320033 |
| Fetal Bovine Serum (FBS) | ThermoFisher | A5256801 |
| Lipofectamine™2000 transfection reagent | Invitrogen™ | 11-668-019 |
| Lipofectamine™ RNAiMAX transfection reagent | Invitrogen™ | 13778100 |
| Paraformaldehyde (PFA) | Delta Microscopies | D15710 |
| Poly-L-lysine (PLL) | Sigma-Aldrich | P8920 |
| PLL(20)-g[3,5]-PEG(2) | SuSoS | |
| Fibrinogen From Human Plasma, Alexa Fluor546 Conjugate | ThermoFisher | F13192 |
| Fibrinogen From Human Plasma, Alexa Fluor647 Conjugate | ThermoFisher | F35200 |
| Polydimethylsiloxane (PDMS) | GE | RTV615 |
| Antibiotic Antimycotic solution (AAs) | ThermoFisher | 15240062 |
| Leibowitz’s L-15 medium | ThermoFisher | 21083027 |
| SiR-DNA | Spirochrome | SC007 |
| Triton™ X-100 | Sigma-Aldrich | X100 |
| DAPI (2-(4-amidinophenyl)-1H -indole-6-carboxamidine) | Sigma-Aldrich | D9542 |
| cOmplete™ Protease Inhibitor Cocktail | Merck | 04693116001 |
| Bradford Protein Assay | Bio-Rad | 5000201 |
| Clarity Western ECL Substrates | Bio-Rad | 1705061 |
| NZYColour Protein Marker II | NYZtech | MB09002 |
| HistoGel™ | Fisher Scientific | 22110678 |
| Tween® 20 | Sigma-Aldrich | P1379 |
| 40% acrylamide/Bis solution 29:1 | Bio-Rad | 1610146 |
| Software | ||
| Fiji image processing software | ImageJ distribution, NIH | |
| Trackosome |
https://github.com/Trackosome/Trackosome Castro et al, 2020 |
|
| MATLAB 2025a | Mathworks | |
| Zen 3 Microscopy software | ZEISS | |
| NIS Elements AR | Nikon | |
| GraphPad Prism 9.5.1 | GraphPad Software | |
| Other | ||
| Static cell confiner | 4DCell | CSOW 620 |
| Dual vacuum/pressure pump | Elveflow | AF1-Dual |
| Dynamic cell confiner | Adapted from (LeBerre et al, 2014) | |
| JEM 1400 transmission electron microscope | JEOL | |
| Deep UV light | Novascan Technologies | PSD-UV |
| Plasma generator | Diener Electronic | Zepto Plasma System |
| Synthetic quartz photomask | Delta Mask | |
| RMC Ultramicrotome | PowerTome | |
| iBlot Gel Transfer Device | Bio-Rad | |