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. Author manuscript; available in PMC: 2006 Mar 27.
Published in final edited form as: Biochemistry. 2005 Sep 27;44(38):12627–12639. doi: 10.1021/bi050832f

Figure 3.

Figure 3

Chemical shift-based identification of structural perturbations in the CaM-smMLCKp complex introduced by various mutations in calmodulin. Shown are superpositions of the 13C-1H chemical shift correlation maps for the wild-type CaM-smMLCKp (black) and E84K-smMLCKp (red); wild-type CaM-smMLCKp (black) and D58N-smMLCKp (green). Many methyl groups throughout the E84K-smMLCKp complex, including all methionines, experience large chemical shift changes as a result of the mutation. D58N-smMLCKp is a more moderate case, where chemical shift perturbations are localized to Ca2+-binding loops I and II and helix C.