Table 1.
Plasmids Constructions and Resulting Polyketide Productsa
| Plasmid | Minimal PKSb | KR(actIII) | Aromatase, Cyclase | R1128 LM | Polyketide Products | Ref |
|---|---|---|---|---|---|---|
| pYT227 | sch with whiE ACP | − | − | − | TW93c-h | Ref. 30 |
| pYT226 | sch with whiE ACP | + | − | − | TW94c-d YT231a, b (1, 2) | Ref. 30 This work |
| pYT232 | sch with whiE ACP | − | − | + | TW93c-h | This work |
| pYT231 | sch with whiE ACP | + | − | + | TW94c-d YT231a, b (1, 2) | This work |
| pYT236 | sch with whiE ACP | − | whiE CYC | + | TW95a, b | Ref. 30 This work |
| pYT210 | sch with zhuN | − | zhuI, zhuJ | + | No polyketide | This work |
| pYT209 | sch with zhuN | + | actVII, actIV | + | No polyketide | This work |
| pYT243 | pms with zhuN | − | − | + | YT243a, b (3, 4) (SEK15-like) | This work |
| pYT266 | pms with zhuN | + | − | + | YT266a, b (5, 6) (RM20b-like) | This work |
| pYT253 | pms with zhuN | + | pms CYC | + | TW95a, b | This work |
| pYT259 | pms with pms ACP | + | pms CYC | + | TW95a, b | This work |
| pYT292 | pms with zhuN | + | grisARO/CYC | + | YT292a, b (7, 8) (SEK43-like) | This work |
| pYT296 | pms with zhuN | + | grisARO/CYC dpsY | + | YT296a, b (9, 10) (aklanonic acid-like) | This work |
| pYT299c | pms with zhuN | + | grisARO/CYC dpsY | + | YT299a, b (11, 12) (methyl ester) | This work |
a
The Streptomyces coelicolor strain CH999 is used as the host for polyketide biosynthesis. Each plasmid is derived from pRM5.16 Each construct contains genes for the minimal PKS, cyclases and the R1128 initiation module, which consists of zhuG, zhuH and zhuC. Products are analyzed by LC/MS and NMR.
b
The minimal PKS consists of the indicated KS-CLF, ACP and the endogenous MAT.
c
pYT299 has methyl transferase(dnrC) gene which is different from pYT296.