Skip to main content
. Author manuscript; available in PMC: 2006 Jan 20.
Published in final edited form as: Exp Dermatol. 2005 Oct;14(10):727–735. doi: 10.1111/j.1600-0625.2005.00351.x

Figure 2. Fate of ex vivo transduced keratinocytes grafted to immunocompetent mice.

Figure 2

(A) Class II expression on mouse epidermal cells as assessed by flow cytometry. Analysis was performed on freshly isolated newborn epidermal cells after staining with isotype matched control antibody (control); anti mouse Ia antibody (EC); anti-mouse Ia antibody following treatment with anti-MHC classII magnetic beads (EC-depleted); and anti-mouse Ia antibody following 3 days of culture (EC-cultured). (B) RT-PCR analysis of APC contamination in epidermal cultures. Total RNA samples from epidermal dendritic cells islolated with Ia-specific immunobeads (DC), freshly isolated epidermal cells (EC), anti-Ia immunobead-depleted cells (EC-depleted), cells cultured for 7 days (EC-cultured) and immortalized mouse keratinocytes by RT-PCR using a set of primers specific for CD11c (upper panel) or phosphoglycerate kinase-1 (lower panel).