Table 1.

Real-time PCR analysis of hTERT, POT1, TERF1 and TERF2 gene expression upon activation in freshly isolated CD4+ T lymphocytes.

	Unstimulated	48 h-Stimulated	Fold Activation
hTERT	0.019*	0.43 ± 0.02	22.6
Pot1	10.16 ± 0.02	3.21 ± 0.05	(-) 3.1
TRF1	2.08 ± 0.08	0.70 ± 0.09	(-) 2.9
TRF2	16.69 ± 8.4	4.24 ± 0.03	(-) 3.9

*Values are corrected for the expression of the human housekeeping gene PBGD in cells of each population. Results are expressed as the amount of indicated mRNA relative to PBGD and compared to that found in Jurkat cells referred as 1. Standard deviations are from two determinations performed in triplicate.