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. Author manuscript; available in PMC: 2026 Aug 5.
Published in final edited form as: Methods Mol Biol. 2026;2969:29–42. doi: 10.1007/978-1-0716-4767-7_3

Table 2.

Thermocycling conditions for dPCR

Step Time Temperature Number of cycles
Heat activation 2 min 95 °C 1×
Denaturation 15 s 95 °C 40×
Annealing/extension 1 min 60 °Ca, b
a

Annealing temperature varies for each assay. Use a temperature gradient to establish optimal annealing temperature

b

The annealing temperature for C-LESS-C1 is 60 °C