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. 2002 Dec;68(12):5918–5924. doi: 10.1128/AEM.68.12.5918-5924.2002

FIG. 3.

FIG. 3.

Detection of lactobacilli in representative ileal content pools, using primer pair Lac1 plus Lac2-GC in a 30 to 45% gradient gel. Lanes: 1 to 4, samples from 7-day-old chickens fed the A− diet (lane 1), the A+ diet (lane 2), the S− diet (lane 3), and the S+ diet (lane 4); 5 to 8, samples from 14-day-old chickens fed the A− diet (lane 5), the A+ diet (lane 6), the S− diet (lane 7), and the S+ diet (lane 8); 9, identification ladder composed of sequences from reference strains of Lactobacillus species (a, L. plantarum; b, L. johnsonii; c, Weissella viridescens; d, L. gasseri; e, L. acidophilus; f, L. crispatus; g, L. salivarius; h, L. ruminis; i, L. rhamnosus; j, L. reuteri); 10 to 13, samples from 21-day-old chickens fed the A− diet (lane 10), the A+ diet (lane 11), the S− diet (lane 12), and the S+ diet (lane 13); 14 to 17, samples from 35-day-old chickens fed the A− diet (lane 14), the A+ diet (lane 15), the S− diet (lane 16), and the S+ diet (lane 17).