FIG. 3.

Single-parameter histograms of cF uptake in O. oeni cells grown without ethanol (a) and in presence of 8% (vol/vol) ethanol (b). Cells were incubated with 50 μM cFDA in potassium phosphate buffer (pH 7) at 30°C. Subsequently, the increase in fluorescence after 15, 30, and 60 min was measured by flow cytometry.