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Flow cytometric analysis of ethanol-induced cF efflux in O. oeni cells. Two-parameter dot plots are shown of cF-loaded cells previously deenergized and incubated with 16% ethanol (vol/vol) at 30°C in potassium phosphate buffer (pH 7). Samples were withdrawn immediately after ethanol addition and after 25 min of incubation. cF retention was analyzed by flow cytometry in nonadapted (a) and ethanol-adapted (b) cells.
