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Flow cytometry analysis of double-stained (PI and cF) O. oeni cells stressed with 16% (vol/vol) ethanol for 25 min. Three main subpopulations of cells were identified in both nonadapted (a) and ethanol-adapted (b) cells, which correspond to cF-stained intact cells (A), cF- and PI-stained cells in an intermediate state of permeability (B), and PI-stained damaged cells (C).
