TABLE 3.
Primer 519r similarity to 16S rRNA sequences in the RDP II database
| Nucleotide | Mismatchesa for the following nucleotide in the 519r primer target (sense, 3′→5′):
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C | A/U | U | A | A | U | G | G | C | G | C | C | G | C/A | C | G | A | C | |
| A | 0/0 | 16/0.8 | 0/0 | 1/0.0 | 0/0 | 0/0 | 0/0 | 2/0.1 | 0/0 | 1/0.0 | 0/0 | 1/0.0 | 1/0.0 | |||||
| T | 1/0.0 | 0/0 | 0/0 | 1/0.0 | 0/0 | 3/0.1 | 0/0 | 12/0.6 | 8/0.4 | 1/0.0 | 2/0.0 | 0/0 | 0/0 | 0/0 | 0/0 | |||
| C | 0/0 | 67/2.8 | 1/0.0 | 4/0.2 | 1/0.0 | 0/0 | 1/0.0 | 2/0.1 | 0/0 | 0/0 | 0/0 | |||||||
| G | 1/0.0 | 0/0 | 87/3.7 | 3/0.1 | 10/0.5 | 0/0 | 1/0.0 | 2/0.1 | 1/0.0 | 2/0.0 | 0/0 | 0/0 | 0/0 | |||||
| Primer 519, reverse (5′→3′) | G | A/T | A | T | T | A | C | C | G | C | G | C | G | G/T | G | C | T | G |
a
Absolute number/percentage of nucleotide-specific mismatches when the primer target consensus sequence was compared against 2,375 16S rRNA sequences retrieved from the RDP II database. (See footnote b, Table 1.)