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. 2002 Dec;68(12):5834–5842. doi: 10.1128/AEM.68.12.5834-5842.2002

FIG. 3.

FIG. 3.

(A) PFGE analysis of XbaI-digested total DNA of STEC O118:[H16] ET-A strains. Lanes: M, molecular weight standard (lambda concatemers; Bio-Rad Laboratories); 1, CB6591 (O118:H12, blaTEM-negative); 2, CB6069 (O118:H12, blaTEM negative); 3, CB6365 (O118:H16, blaTEM-1b); 4, CB6586 (O118:NM, blaTEM-1b); 5, CB6585 (O118:H16, blaTEM-1b); 6, CB6236 (O118:H16, blaTEM-1b); 7, CB5482 (O118:H16, blaTEM-1b); 8, CB6175 (O118:H16, blaTEM-1b). The positions of some XbaI fragments are indicated in kilobases at the left side of the gel. (B) Hybridization of DNA from strains shown in panel A with the blaTEM-specific gene probe. (C) PFGE analysis of XbaI-digested total DNA of STEC O118:[H16] ET-A strains. Lanes: 1, CB6365 (O118:H16, blaTEM-1b); 2, CB7035 (O118:H16, blaTEM-1b); 3, CB7014 (O118:H16, blaTEM-1b); 4, CB6525 (O118:H16, blaTEM-1c); 5, CB6585 (O118:H16, blaTEM-1b); 6, CB6981 (O118:NM, blaTEM-1b); 7, CB6586 (O118:H16, blaTEM-1b); 8, CB7109 (O118:H16, blaTEM-1b); 9, CB6175 (O118:H16, blaTEM-1b). The positions of some XbaI fragments are indicated in kilobases at the left side of the gel. (D) Hybridization of DNA from strains shown in panel A with the blaTEM-specific gene probe.