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. 1975 Sep;182(3):198–206. doi: 10.1097/00000658-197509000-00003

Long term studies of pancreas transplantation in experimental diabetes mellitus.

M J Orloff, S Lee, A C Charters 3rd, D E Grambort, L G Storck, D Knox
PMCID: PMC1343924  PMID: 1099993

Abstract

Alloxan diabetes was induced in inbred rats that then were divided into four groups consisting of unoperated diabetic controls, sham-operated diabetic controls, rats given pancreaticoduodenal isografts, and rats given duct-ligated pancreas isografts. The animals were studied for from 18 months (controls) to two years (transplants) and the following important results were obtained: 1) In striking contrast to the diabetic controls, pancreas transplants of both types produced immediate and permanent relief of hyperglycemia, immediate and lasting elevation of serum insulin levels, a normal weight and growth curve, and good health for two years. Removal of the graft was followed by recurrence of severe diabetes. 2) Pancreas transplants of both types prevented the widespread and severe renal, ophthalmic and neural lesions of diabetes that were found in the diabetic controls. 3) The duct-ligated pancreas graft and pancreaticoduodenal transplant were equally effective in controlling diabetes. Ligation of the pancreatic duct was not followed by significant morphologic or clinical evidence of pancreatitis or by loss of endocrine function. 4) Portal venous drainage of the pancreas transplant was unnecessary for good endocrine function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERGAN J. J., HOEHN J. G., PORTER N., DRY L. TOTAL PANCREATIC ALLOGRAFTS IN PANCREATECTOMIZED DOGS. Arch Surg. 1965 Apr;90:521–526. doi: 10.1001/archsurg.1965.01320100065011. [DOI] [PubMed] [Google Scholar]
  2. Grambort D. E., Lee S., Storck L. G., Chandler J. G., Charters A. C., Elliott M. L., Orloff M. J. Pancreatic duct ligation and long-term endocrine function of pancreas transplants. Surg Forum. 1973;24:299–302. [PubMed] [Google Scholar]
  3. Idezuki Y., Feemster J. A., Dietzman R. H., Lillehei R. C. Experimental pancreaticoduodenal preservation and transplantation. Surg Gynecol Obstet. 1968 May;126(5):1002–1014. [PubMed] [Google Scholar]
  4. Idezuki Y., Goetz F. C., Lillehei R. C. Late effect of pancreatic duct ligation on beta cell function. Am J Surg. 1969 Jan;117(1):33–39. doi: 10.1016/0002-9610(69)90282-7. [DOI] [PubMed] [Google Scholar]
  5. Kyriakides G., Miller J., Lifton J., Najarian J. S. Effect of steroids on structure and endocrine function of duct-ligated porcine pancreatic autografts. Surg Forum. 1974;25(0):384–386. [PubMed] [Google Scholar]
  6. Largiader F., Wegmann W. Experimental orthotopic transplantation of the pancreas. Transplant Proc. 1971 Mar;3(1):497–500. [PubMed] [Google Scholar]
  7. Lee S., Chandler J. G., Krubel R., Nakaji N. T., Rosen H., Orloff M. J. Relief of diabetes mellitus by heterotopic transplantation of the whole pancreas in inbred rats. Surg Forum. 1971;22:75–77. [PubMed] [Google Scholar]
  8. Lee S., Tung K. S., Koopmans H., Chandler J. G., Orloff M. J. Pancreaticoduodenal transplantation in the rat. Transplantation. 1972 Apr;13(4):421–425. doi: 10.1097/00007890-197204000-00010. [DOI] [PubMed] [Google Scholar]
  9. Mauer S. M., Sutherland D. E., Steffes M. W., Leonard R. J., Najarian J. S., Michael A. F., Brown D. M. Pancreatic islet transplantation. Effects on the glomerular lesions of experimental diabetes in the rat. Diabetes. 1974 Sep;23(9):748–753. doi: 10.2337/diab.23.9.748. [DOI] [PubMed] [Google Scholar]
  10. Mitchell R. I., Davidson J. K. Heterotopic autotransplantation of the canine PANCREAS. Surgery. 1967 Sep;62(3):454–461. [PubMed] [Google Scholar]
  11. Rappaport A. M., Vranić M., Wrenshall G. A. A pedunculated subcutaneous autotransplant of an isolated pancreas remnant, for the temporary deprivation of internal pancreatic secretion in the dog. Surgery. 1966 May;59(5):792–798. [PubMed] [Google Scholar]
  12. Rausis C., Choudhury A., Ogata Y. Influence of pancreatic duct anastomosis on function of autotransplanted canine pancreatic segments. J Surg Res. 1970 Nov;10(11):551–557. doi: 10.1016/0022-4804(70)90081-8. [DOI] [PubMed] [Google Scholar]
  13. Sells R. A., Calne R. Y., Hadjiyanakis V., Marshall V. C. Glucose and insulin metabolism after pancreatic transplantation. Br Med J. 1972 Sep 16;3(5828):678–681. doi: 10.1136/bmj.3.5828.678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Teixeira E., Sharkey E., Colwell J., Bergan J. J. Insulin and glucose levels following pancreas allografting. Surg Forum. 1966;17:205–207. [PubMed] [Google Scholar]
  15. Weil R., 3rd, Nozawa M., Koss M., Weber C., Reemtsma K. B., McIntosh R. Effect of pancreas transplantation on diabetic nephropathy in rats. Surg Forum. 1974;25(0):386–388. [PubMed] [Google Scholar]
  16. YALOW R. S., BERSON S. A. Immunoassay of endogenous plasma insulin in man. J Clin Invest. 1960 Jul;39:1157–1175. doi: 10.1172/JCI104130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. de Gruyl J., Verschoor L., Vriesendorp H. M., Horchner P., Westbroek D. L., Hulsmans H. A. DL-A and isolated pancreas allograft. Br J Surg. 1972 Apr;59(4):315–316. [PubMed] [Google Scholar]

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