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. 2002 Dec;68(12):5981–5989. doi: 10.1128/AEM.68.12.5981-5989.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 1. — Freeze tolerance of freeze-resistant strains (AT25, HAT36, HAT43, HAT44) and freeze-sensitive strains (S47 and SS1) used for microarray analysis. IGC, FGC, and RGC were determined 30 min after the onset of fermentation by addition of 100 mM glucose. The cells were either frozen (for 1 day at −30°C) (FGC) or not frozen (i.e., cooled on ice) (IGC). After thawing, glucose consumption was measured for 2.5 h to assess residual yeast activity. RGC is calculated as (FGC/IGC) × 100. Representative results are shown. AT25 showed an RGC 2.0 (±0.3) times higher than that of S47. HAT36, HAT43, and HAT44 each showed an RGC 2.9 (±0.1) times higher than that of SS1.