TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Industrial strains | ||
| S47 | Polyploid, aneuploid, prototrophic | Lesaffre Développement |
| AT25 | Polyploid, aneuploid, prototrophic | 41a |
| SS1 | Polyploid, aneuploid, prototrophic | This study |
| HAT36, -43, -44 | Polyploid, aneuploid, prototrophic | This study |
| S47/HXT7p | S47/pYX012 HXT7p KanMX | This study |
| S47/HXT7pA1-1 | S47/pYX012 HXT7p KanMX AQY1-1 | This study |
| S47/HXT7pA2-1 | S47/pYX012 HXT7p KanMX AQY2-1 | This study |
| AT25/HXT7p | AT25/pYX012 HXT7p KanMX | This study |
| AT25/HXT7pA1-1 | AT25/pYX012 HXT7p KanMX AQY1-1 | This study |
| AT25/HXT7pA2-1 | AT25/pYX012 HXT7p KanMX AQY2-1 | This study |
| AT25/TPIIp | AT25/pYX012 KanMX | This study |
| AT25/TPIIpA2-1 | AT25/pYX012 KanMX AQY2-1 | This study |
| Laboratory strains | ||
| BY4743 | MATa/MATα his3DI leu2D0 ura3D0 | 4 |
| 10560-6B | MATα leu2::hisG trp1::hisG his3::hisG ura3-52 | G. Fink |
| YSH 1170 | 10560-6B aqy1::KanMX4 | 26 |
| YSH 1171 | 10560-6B aqy2::HIS3 | 26 |
| YSH 1172 | 10560-6B aqy1::KanMX4 aqy2::HIS3 | V. Laizé |
| Plasmids | ||
| pUG6 | Containing loxP-KanMX-loxP cassette | 7 |
| pYX012 | Integrative plasmid with TPI promoter, URA3 marker | Novagen |
| pYX012 KanMX | pYX012 URA3::loxP-KanMX-loxP | This study |
| pYX012 KanMX/AQY2-1 | AQY2-1 cloned into pYX012 KanMX | This study |
| pYX012 HXT7p KanMX | pYX012 KanMX TPI1p replaced by HXT7p | This study |
| pYX012 HXT7p KanMX AQY1-1 | AQY1-1 cloned into pYX012 HXT7p KanMX | This study |
| pYX012 HXT7p KanMX AQY2-1 | AQY2-1 cloned into pYX012 HXT7p KanMX | This study |
| pYX242/AQY2-1 | AQY2-1 cloned into pYX242 | 26 |
| pYeDP | 2μm plasmid with GAL10-CYC1 promoter, URA3 marker | 32 |
| pYeDP hAQP1 | hAQP1 (CHIP28) in pYeDP | 19 |
| pYeDP hAQP1-A73M | hAQP1-A73M (CHIP28-A73M) in pYeDP | R. Bill |
| pYeDP KanMX | 2μm plasmid with GAL10-CYC1 promoter, KanMX marker | This study |
| pYeDP hAQP1 KanMX | KanMX in pYeDP hAQP1 | This study |