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SDS-PAGE analysis of the expression and purification of hB4GalT1 and bB4GalT1 WT and their mutants in E. coli Origami B (DE3) as N-terminal MBP fusions. A) MBP_Del1_43 hB4GalT1_His6. B) MBP_Del1_43 bB4GalT1. C) MBP_Del1_43 hB4GalT1-Y285L_His6. D) MBP_Del1_43 bB4GalT1-Y289L_His6. Lanes: M, Precision Plus Protein™ Standard (250 – 10 kD); L, supernatant after lysis; FT, flowthrough from HisTrap™ HP 5 ml column; P, pellet after lysis; E, elution fractions containing the enzyme.
