Scheme 2.

Enzymatic Fc glycan remodeling of trastuzumab to synthesize LacdiNAc terminated antibody glycoform using E. coli expressed B4GalT1 mutants.

Enzymatic Fc glycan remodeling of trastuzumab to synthesize LacdiNAc terminated antibody glycoform using E. coli expressed B4GalT1 mutants.