FIG. 3.

Time course of bioconversion of 2,4,6-TCP, production of 2,4,6-TCA, and growth curves for T. longibrachiatum cultures grown in MEB (A), in MEB supplemented with 111 mM glucose (B), in MEB supplemented with 50 mM ammonium acetate (C), and in MEB containing 111 mM glucose and 50 mM ammonium acetate (D). Growth (•) was estimated by determining mycelial dry weight. Concentrations of 2,4,6-TCP (▴) and 2,4,6-TCA (▵) in supernatants were determined by HPLC. Each data set is the average data for duplicate samples obtained in three independent experiments.