FIG. 4.

Growth curves, levels of 2,4,6-TCP and 2,4,6-TCA, and cell-associated methyltransferase activity in liquid cultures of T. longibrachiatum grown in the presence (A) or in the absence (B) of 2,4,6-TCP (10 μg ml⁻¹). Growth (•) was measured by determining mycelial dry weight. Concentrations of 2,4,6-TCP (▴) and 2,4,6-TCA (▵) in supernatants were determined by HPLC after extraction from culture medium. SAM-dependent methyltransferase activity (○) was estimated by using mycelia. Each data set is the average data for duplicate samples obtained in three independent experiments.