FIG. 2.

MALDI-TOF mass spectrometric analysis of intact whole cells of B. subtilis C-1 grown on agar plates by using Landy (A) and Difco (B) sporulation media and of cells obtained from suspension cultures grown in Landy medium and harvested after 6 h (C) and 30 h (D). In addition, MALDI-TOF mass spectra were obtained for samples of the crude culture filtrate of B. subtilis C-1 grown in Landy medium (E) for 30 h and of the lipopeptide fraction obtained by precipitation with HCl (F). The fermentation temperature for all experiments was 30°C. Lipopeptide biosurfactants were detected in the range from m/z 900 to 1600.