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. 2002 Dec;68(12):6210–6219. doi: 10.1128/AEM.68.12.6210-6219.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — Separation of the biosurfactant products of B. subtilis C-1 on an analytical scale by high-resolution reversed-phase HPLC of a 20-μl aliquot of the methanolic extract of the lipopeptide fraction obtained by precipitation from the culture filtrate with concentrated HCl on a μRPC SC 2.1/10 column by using a Pharmacia Smart microseparation system. (A) HPLC chromatogram. Lipopeptides were eluted with a gradient of 20 to 100% acetonitrile in the presence of 0.1% TFA. (B to D) MALDI mass spectra of aliquots of fractions 25, 30, and 39, respectively.

FIG. 4. — Separation of the biosurfactant products of B. subtilis C-1 on an analytical scale by high-resolution reversed-phase HPLC of a 20-μl aliquot of the methanolic extract of the lipopeptide fraction obtained by precipitation from the culture filtrate with concentrated HCl on a μRPC SC 2.1/10 column by using a Pharmacia Smart microseparation system. (A) HPLC chromatogram. Lipopeptides were eluted with a gradient of 20 to 100% acetonitrile in the presence of 0.1% TFA. (B to D) MALDI mass spectra of aliquots of fractions 25, 30, and 39, respectively.