TABLE 5.

Separation of the lipopeptide biosurfactants produced by B. subtilis C-1 by high-resolution reversed-phase HPLC and analysis by MALDI-TOF mass spectrometry^a

Fraction(s)	Main mass peak(s) (m/z)	Assignment
23, 24	1070.6, 1092.6	C16 iturin, [M+H and Na]+
25	1106.6	C17 iturin, [M+Na]+
26, 27	1120.6	C18 iturin, [M+Na]+
28	1435.8	C14 fengycin, [M+H]+
29	1449.8	C15 fengycin, [M+H]+
30	1463.9, 1485.8	C16 fengycin, [M+H and Na]+
31	1491.8, 1499.8, 1529.7	C16 and C17 fengycins
32	1477.8	C17 fengycin, [M+H]+
	1491.9, 1505.9	C16 and C17 fengycins, [M+H]+
33	1447.9, 1475.9, 1505.9	C15 and C17 fengycins, [M+H]+
34	1461.8, 1475.9	C16 and C17 fengycins, [M+H]+
35	1489.9	C16 fengycin, [M+H]+
36	1475.8, 1497.8	C17 fengycin, [M+H and Na]+
37	1046.6	C13 surfactin, [M+K]+
38	1030.6	C13 surfactin, [M+Na]+
39	1044.6, 1060.5	C14 surfactin, [M+Na and K]+
40	1058.6	C15 surfactin, [M+Na]+
41, 42	1058.7, 1074.6	C15 surfactin, [M+Na and K]+

^aThe mass data represent monoisotopic mass numbers. Twenty microliters of the crude lipopeptide fraction was separated on a μRPC SC 2.1/10 column by using a Pharmacia Smart microseparation system, as described in Materials and Methods.