TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | F−, φ80 lacZΔM15 endA1 recA1 hsdR17(rK− mK+) supE44 thi-1 ΔgyrA96 (ΔlacZYA-argF)U169 relA1 | Lab stocks |
| Burkholderia sp. | ||
| ATCC 25416 | B. cepacia, genomovar I, environmental isolate | D. P. Speert |
| CEP509 | B. cepacia, genomovar I, clinical isolate | D. P. Speert |
| Cep484 | B. multivorans, clinical isolate | D. P. Speert |
| C5424 | B. cepacia, genomovar III, clinical isolate | D. P. Speert |
| C4455 | B. cepacia, genomovar III, clinical isolate | D. P. Speert |
| K56-2 | B. cepacia, genomovar III, clinical isolate | D. P. Speert |
| CEP559 | B. stabilis, clinical isolate | D. P. Speert |
| CEP040 | B. vietnamiensis, clinical isolate | D. P. Speert |
| CEP021 | B. cepacia, genomovar VI, clinical isolate | D. P. Speert |
| LB400 | Burkholderia sp., environmental isolate | S. Sehay |
| Plasmids | ||
| pME6000 | pBBR1 ori, TetrlacZ mob+ | S. Heeb |
| pML50 | pME6000, PS7-dhfr | This study |
| pML51 | pME6000, promoterless dhfr | This study |
| pML52 | pME6000, araC-PBAD-dhfr | This study |
| pBAD24 | AraC AmprcolE1 | D.E. Heinrichs |
| pRK2013 | RK2 derivative, Kmrmob+tra+ ColE1 | 15 |
| pTnMod-OTp′ | Plasposon, Tpr | 13 |
| pMLS7 | pBBR1 ori, PS7 promoter, Tprmob+ | This study |
| pMLBAD | pBBR1 ori, araC-PBAD Tprmob+ | This study |
| pMLS7-eGFP | pMLS7, e-gfp | This study |
| pMLBAD-eGFP | pMLBAD, e-gfp | This study |
| pML53 | pMLBAD-eGFP, PDHFR-araE | This study |
| pIJ8668 | e-gfp Aprr | 40 |
a
Ampr, ampicillin resistance; Aprr, apramycin resistance; Kmr, kanamycin resistance; Tetr, tetracycline resistance; Tpr, trimethoprim resistance.