TABLE 2.
Comparison of fluorescence levels for various strains of the B. cepacia complex associated with pMLS7-eGFP
Strain | Genomovar or species | eGFP expression (rFU)a |
---|---|---|
CEP509 | I | 193 ± 16 |
ATCC25416 | I | 126 ± 17 |
CEP484 | II | 76 ± 6 |
K56-2 | III | 337 ± 9 |
C4455 | III | 75 ± 10 |
C5424 | III | 260 ± 18 |
CEP40 | V | 405 ± 13 |
CEP21 | VI | 207 ± 9 |
LB400 | Burkholderia sp. | 127 ± 15 |
DH5α | E. coli | 16 ± 16 |
Values reflect the mean ± standard deviation of eight determinations. Measurements with strain CEP559 (genomovar IV) were not included since this strain exhibited a high level of autofluorescence that interfered with the detection of eGFP-specific fluorescence.