TABLE 1.

Grouping, based on partial 16S rDNA sequence analysis, cell morphology, and product profiles from glucose fermentation, of isolates from small intestine content of 35-day-old chickens

Identity by 16S rDNA			No. of isolates recovered fromd:						Morphology	Production of organic acidse							Amt of gas (mM)
Isolatea	% Similarb	No. of bpc	CFE	MRS	ROG	SLA	TSC	MW		Total concn (mM)	Molar ratio (%) of:
											F	A	P	B	L	S	H2	CO2
L. aviarius AK113	98	1,091		6	4				Rod	43.0	0.2	3.6	0.0	0.0	96.0	0.2	0.0	18.3
L. salivarius AK21	98	1,354	2	11	6				Rod	43.3	1.8	2.7	0.0	0.0	95.0	0.5	0.0	20.3
E. faecalis AK89	99	943				5			Coccus	49.6	7.0	8.7	0.2	0.0	84.0	0.1	0.0	7.0
E. faecium AK61	99	1,349	10	8		12	1	3	Coccus	44.8	1.6	1.8	0.0	0.0	96.6	0.0	0.0	11.5
C. perfring. AK51	98	1,065					16	16	Rod	33.6	0.0	54.7	5.1	25.7	11.8	2.7	61.3	52.1
S. alactolyt. AK68	99	1,334	4	1					Coccus	32.7	3.2	0.6	0.2	0.0	95.5	0.5	0.0	10.6

^aRepresentative isolates were identified by near-full-length 16S rDNA sequence analysis. Abbreviations: C. perfring., C. perfringens; S. alactolyt., S. alactolyticus.

^bPercentage sequence similarity obtained by comparison to database sequences in GenBank.

^cLength of the near-full-length 16S rDNA sequence.

^dNumber of pure isolates recovered from each medium. Total log₁₀ CFU of bacteria/g of medium were as follows: from CFE, 8.52; from MRS, 7.86; from ROG, 7.07; from SLA, 6.95; from TSC, 5.38; and from MW, 4.88.

^eF, formic acid; A, acetic acid; P, propionic acid; B, butyric acid; L, lactic acid; S, succinic acid.