TABLE 2.
Purification of ADI, OTC, and CK from L. sanfranciscensis CB1
| Enzyme and purification step | Amt of protein (mg) | Total activity (U) | Sp act (U/mg)a | Purification (fold) | Activity yield (%) |
|---|---|---|---|---|---|
| ADI | |||||
| Cytoplasmic extract | 1,370 | 22,198 | 16.20 | 1 | 100 |
| DEAE-cellulose | 141 | 12,320 | 87.37 | 5.39 | 55.50 |
| Superose 12 | 17.1 | 4,031 | 235.73 | 14.55 | 18.15 |
| Phenyl-Superose | 10.2 | 2,838 | 278.23 | 17.17 | 12.78 |
| Mono-Q | 1.8 | 1,064 | 591.11 | 36.48 | 4.79 |
| OTC | |||||
| Cytoplasmic extract | 1,370 | 41,530 | 30.31 | 1 | 100 |
| DEAE-cellulose | 24.2 | 6,221 | 257.06 | 8.48 | 14.98 |
| Superose 12 | 0.47 | 385 | 818.72 | 27.01 | 0.92 |
| Precipitation by (NH4)2SO4 | 0.12 | 201 | 1,675 | 55.26 | 0.48 |
| Mono-Q | 0.07 | 137 | 1,960 | 64.66 | 0.33 |
| CK | |||||
| Cytoplasmic extract | 1,370 | 1,461 | 1.06 | 1 | 100 |
| DEAE-cellulose | 37.4 | 231 | 6.17 | 5.82 | 15.79 |
| Superose 12 | 1.83 | 28 | 15.08 | 14.22 | 1.89 |
| Phenyl-Superose | 0.51 | 15 | 30 | 28.30 | 1.04 |
| Mono-Q | 0.12 | 7 | 60 | 56.60 | 0.49 |
| Mono-Q | 0.03 | 2 | 80 | 75.47 | 0.16 |
a
For determination of specific activity, see Materials and Methods.