TABLE 3.
Effect of chemical reagents and divalent cations on the activities of ADI, OTC, and CK from L. sanfranciscensis CB1
| Inhibitor or cation (2 mM) | Relative activitya (%)
|
||
|---|---|---|---|
| ADI | OTC | CK | |
| NEM | 90D | 140A | 10L |
| Iodacetamide | 84E | 100D | 15I |
| Cysteine | 127A | 110C | 108AB |
| PMSF | 0H | 0M | 91C |
| EDTA | 103C | 40I | 20H |
| dl-Penicillamine | 88E | 58G | 100B |
| dl-Propargylglycine | 121A | 93E | 0N |
| DTT | 87D | 46H | 110A |
| Mg2+ | 98C | 100D | 100B |
| Cu2+ | 0H | 100D | 85D |
| Co2+ | 100C | 100D | 30G |
| Hg2+ | 12G | 71F | 20H |
| Ni2+ | 91D | 33L | 4M |
| Mn2+ | 116B | 122B | 90C |
| Ca2+ | 109B | 100D | 105B |
| Fe2+ | 83E | 0M | 35F |
| Zn2+ | 23F | 100D | 40E |
a
The activity of the control in the absence of chemical reagents and divalent cations was taken as 100%. Values in the same column without a common uppercase letter are significantly different (P < 0.05).