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Array washing conditions for each OFRG probea
| Probe no. | Washing buffer (× SSC) | Washing time (min) |
|---|---|---|
| 1 | 1 | 30 |
| 2 | 1 | 30 |
| 3 | 1 | 30 |
| 4 | 4 | 15 |
| 5 | 0.1 | 30 |
| 6 | 0.1 | 30 |
| 7 | 4 | 15 |
| 8 | 1 | 30 |
| 9 | 1 | 30 |
| 10 | 1 | 30 |
| 11 | 0.1 | 30 |
| 12 | 0.1 | 30 |
| 13 | 1 | 15 |
| 14 | 0.1 | 30 |
| 15 | 1 | 30 |
| 16 | 0.1 | 30 |
| 17 | 1 | 15 |
| 18 | 4 | 15 |
| 19 | 1 | 30 |
| 20 | 0.1 | 30 |
| 21 | 1 | 15 |
| 22 | 1 | 15 |
| 23 | 1 | 15 |
| 24 | 4 | 15 |
| 25 | 1 | 15 |
| 26 | 4 | 15 |
| 27 | 1 | 30 |
After hybridization with the 33P-labeled probes, arrays were washed twice with the buffers and for the times indicated
