TABLE 4.
13C-PLFA profiles of phenanthrene-degrading soil organisms in Risø, Amager, B&W, and Ringe soils and PLFA compositions of phenanthrene-degrading laboratory reference strains
| Fatty acid |
13C-PLFA abundance (%) in soilsa
|
PLFA abundance (%) in reference strainsa,b
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Risø | Amager | B&W | Ringe | LH128 | EPA505 | JAJ137 | FAn9 | VM552 | VM451 | ARJ45 | |
| Unidentified 12 | 3.5 | 1.0 | |||||||||
| 14:0 | 4.7 | 3.0 | 0.7c | 4.7 | 4.6 | 3.2 | |||||
| br15:0 | 2.2 | 1.4 | 1.0 | 0.8c | 1.0 | ||||||
| Unidentified | 1.0 | ||||||||||
| 16:1ω9 | 1.9 | ||||||||||
| 16:1ω7/ω5 | 16.1 | 12.0 | 31.3 | 36.1 | 20.6 | 12.7 | 48.4 | 4.8 | 19.5 | 16.9 | 15.6 |
| 16:0 | 42.4 | 44.6 | 24.7 | 26.5 | 2.8 | 6.2 | 35.2 | 20.5 | 23.2 | 24.3 | 34.0 |
| me16:0 | 1.8 | ||||||||||
| Unidentified 17 | 2.4 | ||||||||||
| 17:1 | 14.8 | 14.1 | 46.2 | ||||||||
| 18:1ω9 | 11.1 | 18.0 | 17.4 | 32.7 | 26.7 | 1.9 | |||||
| 18:1ω7/ω5 | 21.0 | 5.4 | 17.1 | 20.2 | 65.7 | 71.9 | 15.3 | ||||
| 18:0 | 1.1 | 29.4 | 1.5 | ||||||||
| br19:1 | 4.3 | 2.0 | |||||||||
| 10/12me18:0 | 7.4 | 18.4 | 27.1 | ||||||||
| Unidentified | 1.0 | ||||||||||
| 19:1 | 7.6 | 1.2 | 3.0 | ||||||||
| 20:0 | 23.3 | ||||||||||
| Other | 0.6 | 5.8 | 3.6 | 0.4 | 2.2 | 1.1 | 1.1 | 0.3 | |||
a
The PLFA abundance is the relative amount of 13C (soils) or total C (reference strains) in each fatty acid.
b
The identities of the reference strains are as follows: LH128, Sphingomonas sp.; EPA505, S. paucimobilis; JAJ137, P. fluorescens; FAn9, M. frederiksbergense; VM552, M. gilvum; VM451, N. asteroides; and ARJ45, unclassified beta-proteobacterium isolated in this study.
c
PLFAs highly enriched in 13C but containing less than 1% of the total amount of 13C due to small amounts of the PLFAs.