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Degradation of γ-HCH by E. coli BL21(pLINEA1) or BL21(pGEX-5X-3). (a) Conversion of 5 μg of γ-HCH/ml by pLINEA1 and pLINEA2 in E. coli BL21 growing in LB medium. Samples were drawn periodically and analyzed by gas chromatography. ▴, control; ♦, pLINEA1; ▪, pLINEA2. (b) Degradation of γ-HCH by cell extracts of E. coli BL21(pLINEA1) expressing the linA1 gene. The cell extracts contained 5 mg of protein/ml, to which 5 μg of γ-HCH/ml was added. ▴, control; ♦, pLINEA1; ▪, pLINEA2.
