TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Sourceb |
|---|---|---|
| E. coli DH5α | F−endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 ΔU169 deoR | Laboratory stock |
| E. coli BL21 | B F−ompT hsdS (rB− mB−) gal dcm | Amersham Pharmacia |
| E. coli JM101 | supE thi Δ(lac-proAB) F′[tra D36 proAB+lacIqlacZ ΔM15] | Laboratory stock |
| S. paucimobilis B90 | Aerobic, motile, rod shaped; degrades all four isomers of HCH (α, β, γ, and δ) | CRRI, Cuttack, India |
| S. paucimobilis UT26 | Aerobic, motile, rod shaped; degrades α, γ, and δ-HCH but not β-HCH | Y. Nagata |
| pUC 13/19 | 2.7-kb; Ampr; MCS internal to lacZ gene | Laboratory stock |
| pGEX-5X-3 | GST fusion vector with tac promoter, lacIq, factor Xa protease recognition site | Amersham Pharmacia |
| pIMA2 | Recombinant construct carrying truncated linA gene | Y. Nagata |
| pYNA4R | Recombinant construct carrying truncated linB gene | Y. Nagata |
| pBFRU2 | Recombinant construct carrying truncated linC gene | Y. Nagata |
| pKM2 | Recombinant construct carrying truncated linD gene | Y. Nagata |
| pLIN14 | pUC13 carrying 1.4-kb BclI fragment from genomic DNA of S. paucimobilis B90 | This study |
| pLIN25 | pUC13 carrying 2.5-kb BclI fragment from genomic DNA of S. paucimobilis B90 | This study |
| pLINA1 | pUC19 containing linA1 gene | This study |
| pLINA2 | pUC19 containing linA2 gene | This study |
| pLINB | pUC19 containing linB gene | This study |
| pLINC | pUC19 containing linC gene | This study |
| pLIND | pUC19 containing linD gene | This study |
| pLINX | pUC19 containing linX gene | This study |
| pLINEA1 | pGEX-5X-3 containing linA1 gene | This study |
| pLINEA2 | pGEX-5X-3 containing linA2 gene | This study |
a
MCS, multiple cloning site; GST, glutathione S-transferase.
b
CRRI, Central Rice Research Institute.