TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′-3′)a | Added restriction site | Designation | Source or accession no. |
|---|---|---|---|---|
| 1 | GCGGATCCGCATGAGTGATCTAGACAGACTT | BamHI | linA-sense | D90355 |
| 2 | GCCTCGAGTTATGCGCCGGACGGTGCGAAATG | XhoI | linA1-antisense | This study |
| 3 | GCCTCGAGTCACGATTTTTGCAACAGAGC | XhoI | linA2-antisense | This study |
| 4 | GCGGATCCGCATGAGCCTCGGCGCAAAGCCA | BamHI | linB-sense | D14594 |
| 5 | GCCTCGAGTTATGCTGGGCGCAATCGCCGGAC | XhoI | linB-antisense | D14594 |
| 6 | GCGGATCCGCATGTCTGATTTGAGCGGC | EcoRI | linC-sense | D14595 |
| 7 | GCCTCGAGTCAGATCGCGGTAAAGCCGCCGTC | XhoI | linC-antisense | D14595 |
| 8 | GCGAATTCAATGAGCGCTGATACAGAA | EcoRI | linD-sense | D89733 |
| 9 | GCCTCGAGTTAGGCGTTGCTCAGGAGATGGAT | XhoI | linD-antisense | D89733 |
| 10 | GCGGATCCGCATGGCTAACAGACTCGCAGGCA | BamHI | linX-sense | D23722 |
| 11 | GCCTCGAGTCAAACACCCACGGACCAGCCTCC | XhoI | linX-antisense | D23722 |
| 12 | AGGAATTCCATGATGCAACTGCCCGAA | EcoRI | linE-sense | AB021867 |
| 13 | AGCTCGAGCTCAAATGACGATCGGATC | XhoI | linE-antisense | AB021867 |
| 14 | TGGGATCCCCGTGAATATAGATGACCTGG | BamHI | linR-sense | AB021860 |
| 15 | GGGTCGACTCACATCCGCGCGGACAG | SalI | linR-antisense | AB021860 |
| 16 | GCGGATCCGCTCTGTTGCAAAAATCGTGAAGC | BamHI | Tn610-sense | X536535 |
| 17 | GCGGATCCGATGACCATGATTACGCC | BamHI | Tn610-antisense | X536535 |
a
Restriction sites are underlined.