TABLE 1.
Stoichiometry of successive generations of a genealogy of lysine-producing C. glutamicum strains during lysine production phase
| Product | Yield for C. glutamicuma:
|
||||
|---|---|---|---|---|---|
| ATCC 13032 | ATCC 13287 | ATCC 21253 | ATCC 21526 | ATCC 21543 | |
| Biomass | 60.65 ± 1.94 | 43.26 ± 1.10 | 39.40 ± 1.00 | 42.84 ± 1.05 | 41.35 ± 0.10 |
| Lysine | 11.84 ± 0.09 | 173.13 ± 5.75 | 199.85 ± 6.64 | 183.67 ± 6.86 | 249.15 ± 6.20 |
| Valine | 50.26 ± 0.70 | 6.71 ± 0.33 | 23.07 ± 1.12 | 19.96 ± 0.49 | 19.90 ± 0.68 |
| Alanine | 41.01 ± 2.09 | 7.49 ± 0.17 | 12.55 ± 0.29 | 16.72 ± 0.66 | 10.57 ± 0.67 |
| Glycine | 7.86 ± 0.34 | 29.67 ± 1.25 | 31.45 ± 1.33 | 4.94 ± 0.21 | 48.37 ± 3.98 |
| Glutamate | 0.32 ± 0.15 | 0.24 ± 0.02 | 0.23 ± 0.02 | 0.00 ± 0.00 | 0.22 ± 0.04 |
| Glycerol | 47.55 ± 2.34 | 31.23 ± 0.28 | 29.75 ± 0.27 | 56.91 ± 3.70 | 31.51 ± 2.39 |
| Trehalose | 5.04 ± 0.18 | 11.57 ± 1.03 | 14.71 ± 1.31 | 14.35 ± 1.13 | 12.48 ± 1.05 |
| α-Ketoglutarate | 7.50 ± 0.68 | 3.12 ± 0.33 | 13.42 ± 1.40 | 5.41 ± 0.13 | 6.60 ± 0.64 |
| Succinate | 25.15 ± 3.60 | 5.44 ± 0.43 | 35.64 ± 2.81 | 0.33 ± 0.00 | 16.56 ± 1.06 |
| Acetate | 24.66 ± 0.21 | 10.59 ± 0.03 | 14.57 ± 0.04 | 24.02 ± 0.89 | 6.39 ± 0.85 |
| Lactate | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 35.34 ± 4.87 | 1.21 ± 0.18 |
| Pyruvate | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 19.29 ± 0.03 | 0.00 ± 0.00 |
a
Experimental data are given as mean values of two parallel incubations on (i) [1-13C]glucose and (ii) a 1:1 mixture of naturally labeled and [13C6]glucose with corresponding deviations between the two incubations. All yields are given in millimoles of product moles of glucose−1 except the yield for biomass, which is given in milligrams of dry biomass millimoles of glucose−1.