TABLE 2.

¹³C mass isotopomer ratios of secreted products alanine, valine, lysine, and trehalose in successive generations of a genealogy of lysine-producing C. glutamicum strains cultivated on [1-¹³C]glucose

Analyte	Mass isotopomer ratio	Source	Value for C. glutamicuma:
			ATCC 13032	ATCC 13287	ATCC 21253	ATCC 21526	ATCC 21543
Alanine	m + 1/m	Exp	0.75 ± 0.02	0.73 ± 0.02	0.70 ± 0.02	0.70 ± 0.02	0.67 ± 0.02
		Calc	0.74	0.69	0.72	0.68	0.64
	m + 2/m	Exp	0.24 ± 0.01	0.22 ± 0.01	0.20 ± 0.01	0.20 ± 0.00	0.19 ± 0.01
		Calc	0.23	0.21	0.22	0.21	0.20
	m + 2/m + 1	Exp	0.32 ± 0.00	0.30 ± 0.01	0.28 ± 0.00	0.29 ± 0.01	0.29 ± 0.01
		Calc	0.31	0.31	0.31	0.31	0.31
Valine	m + 1/m	Exp	1.24 ± 0.01	1.16 ± 0.01	1.19 ± 0.00	1.18 ± 0.04	1.06 ± 0.04
		Calc	1.24	1.14	1.19	1.12	1.04
	m + 2/m	Exp	0.62 ± 0.01	0.56 ± 0.01	0.57 ± 0.01	0.57 ± 0.02	0.48 ± 0.02
		Calc	0.61	0.53	0.57	0.52	0.46
	m + 2/m + 1	Exp	0.50 ± 0.01	0.48 ± 0.01	0.48 ± 0.01	0.48 ± 0.01	0.45 ± 0.01
		Calc	0.49	0.47	0.48	0.46	0.44
Lysine	m + 1/m	Exp	1.70 ± 0.03	1.57 ± 0.02	1.57 ± 0.04	1.55 ± 0.01	1.46 ± 0.01
		Calc	1.72	1.59	1.63	1.56	1.46
	m + 2/m	Exp	1.24 ± 0.04	1.12 ± 0.01	1.11 ± 0.03	1.08 ± 0.04	0.98 ± 0.02
		Calc	1.31	1.14	1.19	1.10	0.98
	m + 2/m + 1	Exp	0.73 ± 0.02	0.72 ± 0.01	0.71 ± 0.01	0.70 ± 0.03	0.67 ± 0.01
		Calc	0.76	0.71	0.73	0.70	0.67
Trehalose	m + 2/m + 1	Exp	2.64 ± 0.13	2.39 ± 0.12	2.22 ± 0.11	2.36 ± 0.12	2.23 ± 0.11
		Calc	2.72	2.47	2.30	2.37	2.32

^aExperimental data with standard deviation (Exp) obtained by GC-MS (for TBDMS-derivatized alanine, valine, and lysine) or MALDI-TOF MS (for trehalose) and values predicted by the solution of the mathematical model corresponding to the optimized set of fluxes (Calc).