TABLE 3.

¹³C mass isotopomer ratios of secreted products alanine, valine, and lysine in successive generations of a genealogy of lysine-producing C. glutamicum strains cultivated on a 1:1 mixture of naturally labeled and [¹³C₆]glucose

Analyte	Mass isotopomer ratio	Source	Value for C. glutamicuma:
			ATCC 13032	ATCC 13287	ATCC 21253	ATCC 21526	ATCC 21543
Alanine	m + 1/m	Exp	0.43 ± 0.02	0.43 ± 0.01	0.41 ± 0.00	0.41 ± 0.01	0.40 ± 0.02
		Calc	0.41	0.40	0.40	0.41	0.40
	m + 2/m	Exp	0.35 ± 0.01	0.35 ± 0.01	0.35 ± 0.01	0.34 ± 0.01	0.33 ± 0.01
		Calc	0.33	0.32	0.32	0.33	0.32
	m + 2/m + 1	Exp	0.81 ± 0.01	0.81 ± 0.03	0.86 ± 0.02	0.86 ± 0.03	0.84 ± 0.02
		Calc	0.80	0.80	0.80	0.81	0.80
Valine	m + 1/m	Exp	0.59 ± 0.01	0.55 ± 0.01	0.57 ± 0.01	0.56 ± 0.02	0.55 ± 0.01
		Calc	0.60	0.59	0.58	0.59	0.57
	m + 2/m	Exp	1.29 ± 0.01	1.29 ± 0.03	1.24 ± 0.01	1.27 ± 0.04	1.33 ± 0.02
		Calc	1.28	1.27	1.27	1.32	1.27
	m + 2/m + 1	Exp	2.20 ± 0.02	2.34 ± 0.04	2.18 ± 0.05	2.24 ± 0.05	2.41 ± 0.04
		Calc	2.15	2.17	2.18	2.23	2.24
Lysine	m + 1/m	Exp	1.61 ± 0.05	1.58 ± 0.01	1.46 ± 0.02	1.50 ± 0.03	1.43 ± 0.02
		Calc	1.55	1.58	1.48	1.52	1.42
	m + 2/m	Exp	2.44 ± 0.04	2.41 ± 0.01	2.25 ± 0.04	2.32 ± 0.03	2.14 ± 0.06
		Calc	2.38	2.41	2.24	2.30	2.13
	m + 2/m + 1	Exp	1.51 ± 0.02	1.53 ± 0.00	1.54 ± 0.04	1.54 ± 0.03	1.50 ± 0.02
		Calc	1.53	1.53	1.51	1.51	1.50

^aExperimental data with standard deviation (Exp) obtained after TBDMS derivatization by GC-MS and values predicted by the solution of the mathematical model corresponding to the optimized set of fluxes (Calc).