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. 2002 Dec;68(12):5843–5859. doi: 10.1128/AEM.68.12.5843-5859.2002

TABLE 5.

Statistical evaluation of comparative metabolic flux analysis of a genealogy of lysine-producing C. glutamicum strains by 13C tracer studies with MS and metabolite balancing

Flux parameter Value for C. glutamicuma:
ATCC 13032 ATCC 13287 ATCC 21253 ATCC 21526 ATCC 21543
Fluxes
    Glucose 6-phosphate dehydrogenase (ν3) [49.6    52.9] [55.3    58.7] [53.4    57.5] [56.6    61.0] [61.6    65.8]
    Glucose 6-phosphate isomerase (ν5 − ν6) [44.8    48.2] [38.1    41.5] [38.8    42.8] [35.2    39.7] [30.9    35.1]
    Aldolase [75.5    76.8] [73.9    75.2] [74.1    75.6] [72.6    74.3] [71.6    73.1]
    Pyruvate kinase (ν20) [151.0    153.6] [152.8    154.9] [153.0    155.9] [150.8    153.6] [148.8    151.4]
    Pyruvate dehydrogenase (ν28) [97.0    104.8] [90.8    96.0] [81.1    84.7] [78.2    84.4] [68.4    73.4]
    Pyruvate carboxylase (ν30) [45.6    55.7] [52.7    62.1] [58.1    65.6] [54.1    64.8] [55.4    64.5]
    Phosphoenolpyruvate carboxykinase (ν31) [25.7    31.8] [22.3    30.7] [25.1    32.6] [22.9    33.1] [22.9    33.1]
    Isocitrate dehydrogenase (ν33) [77.0    87.2] [78.4    84.5] [69.6    73.6] [64.3    71.7] [57.2    62.4]
    Isocitrate lyase (ν34) [0.0    3.6] [0.0    0.4] [0.0    0.0] [0.0    0.9] [0.0    0.9]
    Lysine synthetic pathway (ν44) [3.2    3.4] [17.8    19.8] [21.0    21.7] [18.6    21.0] [25.4    27.4]
    Succinylase branch of lysine synthesis (ν45) [1.1    1.5] [14.2    15.7] [14.9    19.4] [13.0    18.7] [19.0    18.7]
    Dehydrogenase branch of lysine synthesis (ν46) [0.2    1.1] [3.3    4.4] [1.9    6.5] [1.1    6.9] [1.0    7.5]
Flux partitioning ratios
    ΦPPP [ν3/(ν3 + ν5 − ν6)] [0.51    0.54] [0.57    0.61] [0.56    0.60] [0.59    0.63] [0.64    0.68]
    ΦPC [(ν30 − ν31)/(ν30 − ν31 + ν28)] [0.15    0.20] [0.24    0.26] [0.28    0.29] [0.26    0.29] [0.34    0.37]
    ΦDH (ν46/(ν45 + ν46) [0.07    0.33] [0.18    0.23] [0.09    0.30] [0.06    0.34] [0.04    0.28]
Flux reversibilities
    ζPGI[(ν6/(ν5 − ν6)] [3.5    11.7] [4.2    9.7] [5.7    23.7] [3.4    11.8] [4.1    8.6]
    ζPC/PEPCK [(ν31/(ν30 − ν31)] [1.1    1.6] [0.7    1.0] [0.8    1.0] [0.7    1.0] [0.4    0.6]
    ζTCA [(ν42/ν41 − ν42)] [10.2    25.0] [0.7    3.1] [0.3    25.0] [0.0    18.8] [7.1    25.0]
a

Mean values and 90% confidence intervals of flux parameters obtained by a Monte Carlo approach, including 250 independent parameter estimation runs with varied experimental data for each strain. The flux numbers according to Fig. 1 are given for the examined reactions.