TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristicsa | Source or reference(s) |
|---|---|---|
| Strains | ||
| A. baumannii | ||
| 9235, 8971, 8143, 8114, 8637, 7133, 7138, 8399, 9606, 7931, 9124, 9397 | Clinical isolates representing plasmid profiles A to K and W, respectively | 2, 22 |
| BM4420, BM4421, BM4422, BM4424, BM4427, BM4430, BM4432, BM4436, BM4439 | Clinical isolates representing RAPD types A to F, H, I, and K, respectively | 46 |
| E. coli | ||
| DH5α | Used for recombinant DNA methods | Gibco-BRL (Gaithersburg, Md.) |
| TransforMax EC100D | pir+, used to clone R6K derivatives | Epicentre (Madison, Wis.) |
| CC118, SM10, and S17-1 | λpir, used to maintain and conjugate suicide plasmid pLOFKm | 10, 26 |
| Plasmids | ||
| pLOFKm | Suicide plasmid harboring mini-Tn10Km; Ampr Kmr | 26 |
| pVK100 | Cosmid cloning vector; Kmr Tetr | 33 |
| pRK2073 | Conjugation helper plasmid | 37 |
| pWH1266 | E. coli-Acinetobacter shuttle plasmid; Ampr Tetr | 28 |
| pFVP25 | ColEI, mob+, Ampr; source of the BamHI-PstI fragment harboring gfp | 56 |
| pBCSK+ | Cloning vector; Cmr | Stratagene (La Jolla, Calif.) |
| pMU125 | pWH1266 with gfp under control of the Tet promoter | This work |
a
Ampr, ampicillin resistant; Kmr, kanamycin resistant; Tetr, tetracycline resistant; RAPD, random amplified polymorphic DNA.